Perfect upgrade on quality,new breakthroughs in Click Chemistry Series Product

1. A heterobifunctional peg bioorthogonal linker with azide (click reaction) and amine reactivity
The advent of bioorthogonal chemical biology tools for imaging and tracking of biomolecules (proteins, lipids, glycans) in their native environment is providing unique insights into cellular processes that are not achievable with traditional biochemical or molecular biology tools. However, the use of copper in traditional click labeling chemistry limits its application in chemistry biology research wherein oligonucleotide or polysaccharide involved could be degraded by copper. The advancement of metal-free bioorthogonal cycloaddtions between strain-promoted alkyne, so called cyclooctynes, with azide (SPAAC), tetrazines or nitrones (SPANC) could solve this issue. Among the cyclooctyned invented, bicyclo[6.1.0]nonyne (BCN) featuring Cs symmetry displays excellent cycloaddition reaction kinetics.

Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)

Chemoselective – azides and BCN groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency.

Amine reactivity – modify amine-containing molecules or biomolecules with BCN moiety for subsequent conjugation/labelling with azide counterpart.

2. Copper-free click click reagents is a bioorthogonal reaction developed as an activated variant of an azide alkyne Huisgen cycloaddition. Cu-free click chemistry has been modified to be bioorthogonal by eliminating a cytotoxic copper catalyst, allowing reaction to proceed quickly and without live cell toxicity. Although the reaction produces a regioisomeric mixture of triazoles, the lack of regioselectivity in the reaction is not a major concern for its applications in bioorthogonal chemistry. More regiospecific and less bioorthogonal requirements are best served by the traditional Huisgen cycloaddition, especially given the low yield and synthetic difficulty (compared to the addition of a terminal alkyne) of synthesizing a strained cyclooctyne. The incredible bioorthogonality of the reaction has allowed the Cu-free click reaction to be applied within cultured cells, live zebrafish, and mice.

Dibenzylcyclooctyne (DBCO) is widely used in the Cu-free click chemistry. DBCO eliminates the use of cytotoxic copper catalyst, and allows reaction to proceed quickly and without live cell toxicity.
This acid functionalized cyclooctyne derivative is useful in strain-promoted copper-free azide-alkyne cycloaddition reactions. This dibenzocyclooctyne will react with azide functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage.

Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)

Chemoselective – azides and DBCO groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency

Primary amine reactivity – modify amine-containing molecules with DBCO for use in advanced crosslinking experiments.

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